A crash involving a SunRail train and an SUV brought the train to a halt at Michigan Street, south of downtown Orlando.
No one was injured in the SUV, or on the SunRail train, according to Orlando fire officials at the scene.
Orlando police said the crash was minor, and a tow truck was called to remove the vehicle.
The train completely blocked Michigan Street between Division Avenue and South Orange Avenue, south of downtown Orlando, as firefighters investigated.
The roadway is now clear.
Friday's crash is the third involving a SunRail train since the commuter rail line went into service in May. In both previous incidents, a vehicle was stopped on the train tracks when it was hit by a train.